Fertility and Sterility, 89(2), 375-379
To evaluate the effect of vaginal lubricants Pre-Seed, FemGlide, Astroglide, and Replens on human sperm motility and chromatin integrity.
Prospective, comparative, in vitro study.
Andrology laboratory at tertiary care hospital.
PATIENT(S): Thirteen normozoospermic donors.
INTERVENTION(S): Semen samples from 13 subjects were incubated in human tubal fluid media (HTF) controls and 10% (vol/vol) of Pre-Seed, FemGlide, Astroglide, and Replens lubricants. After 30 minutes, progressive sperm motility was assessed by light microscopy. Semen samples of 12 patients were placed in positive control (HTF), negative control (10% K-Y Jelly lubricant), and 10% vol/vol Pre-Seed and FemGlide lubricants. After 4 hours culture, spermatozoa were analyzed for percent DNA fragmentation index with use of the acridine orange-based sperm chromatin structure assay.
MAIN OUTCOME MEASURE(S): Sperm motility and percent DNA fragmentation index.
Percent motility did not differ significantly between HTF controls and Pre-Seed, whereas FemGlide, Replens, and Astroglide lubricants demonstrated a significant decrease in motility. There was no significant difference in percent DNA fragmentation index between the HTF controls and Pre-Seed, but a significant decline in sperm chromatin quality occurred with FemGlide and K-Y Jelly.
Pre-Seed does not cause a significant decrease in progressive sperm motility or chromatin integrity in contrast to other lubricants used by couples.
Agarwal, A., Deepinder, F., Cocuzza, M., Short, R. A., & Evenson, D. P. (2008). Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study. *Fertility and sterility*, *89*(2), 375-379. https://doi.org/10.1016/j.fertnstert.2007.02.050
Agarwal A, Deepinder F, Cocuzza M, Short RA, Evenson DP. Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study. Fertil Steril. 2008;89(2):375-379. doi:10.1016/j.fertnstert.2007.02.050
Agarwal, A., et al. "Effect of vaginal lubricants on sperm motility and chromatin integrity: a prospective comparative study." *Fertility and sterility*, vol. 89, no. 2, 2008, pp. 375-379.
Cells, Cultured, Cellulose/analogs & Derivatives/pharmacology, Chromatin/drug Effects/metabolism, DNA Fragmentation/drug Effects, Female, Glycerol/pharmacology, Humans, Lipids/pharmacology, Lubricants/pharmacology, Male, Phosphates/pharmacology, Poloxamer/pharmacology, Polyethylene Glycols/pharmacology, Propylene Glycols/pharmacology, Sperm Motility/drug Effects, Spermatozoa/drug Effects/metabolism, Vaginal Creams, Foams, and Jellies/pharmacology, Astroglide, Chromatin, K-Y Jelly, Lipids, Lubricants, Phosphates, Propylene Glycols, Replens, Vaginal Creams, Foams, and Jellies, Femglide, Pre-seed, Poloxamer, Polyethylene Glycols, Cellulose, Glycerol,