American Journal of Obstetrics and Gynecology, 101(8), 1054-1057
A method to measure plasma progesterone by gas-liquid chromatography is presented. The procedure allows for the preparation of a plasma progesterone extract clean enough for accurate submicrogram quantification by gas-liquid chromatography. In a gas chromatograph equipped with a hydrogen flame detector, the instrument modifications and operating conditions as outlined make possible the measurement of 0.001 μg (1 ng.) of progesterone standard on a stable recorder base line. In biologic samples the sensitivity of the assay is in the order of 0.01 μg (10 ng.) of progesterone.
Yannone, M. E. (1968). Plasma progesterone levels in normal pregnancy, labor, and the puerperium. I. Method of assay. *American journal of obstetrics and gynecology*, *101*(8), 1054-1057. https://doi.org/10.1016/0002-9378(68)90347-5
Yannone ME. Plasma progesterone levels in normal pregnancy, labor, and the puerperium. I. Method of assay. Am J Obstet Gynecol. 1968;101(8):1054-1057. doi:10.1016/0002-9378(68)90347-5
Yannone, Michael E. "Plasma progesterone levels in normal pregnancy, labor, and the puerperium. I. Method of assay." *American journal of obstetrics and gynecology*, vol. 101, no. 8, 1968, pp. 1054-1057.
Chromatography, Gas, Chromatography, Paper, Female, Labor, Obstetric, Plasma/enzymology, Postpartum Period, Pregnancy, Progesterone/blood, Progesterone