Acta Endocrinologica, 45(3), 321-328
Gonadotrophins were prepared from human postmenopausal urine with the kaolin-acetone method and purified by adsorption of impurities on diethylamine ethyl cellulose (DEAE-C).
The last step, namely chromatography on the column of permutit, resulted in a significantly improved specific activity.
The immunoelectrophoresis showed that the purified HMG is not homogeneous and that the constituents are glycoproteins which migrate into the region α1, α2and β globulins.
Donini, P., Puzzuoli, D., & Montezemolo, R. (1964). Purification of gonadotrophin from human menopausal urine. *Acta endocrinologica*, *45*(3), 321-328. https://doi.org/10.1530/acta.0.0450321
Donini P, Puzzuoli D, Montezemolo R. Purification of gonadotrophin from human menopausal urine. Acta Endocrinol (Copenh). 1964;45(3):321-328. doi:10.1530/acta.0.0450321
Donini, P., et al. "Purification of gonadotrophin from human menopausal urine." *Acta endocrinologica*, vol. 45, no. 3, 1964, pp. 321-328.
Alpha-Globulins, Beta-Globulins, Chromatography, Glycoproteins, Gonadotropins, Gonadotropins, Pituitary, Immunoelectrophoresis, Menopause, Serum Globulins, Urine, Alpha-Globulins, Beta-Globulins, Glycoproteins, Gonadotropins, Gonadotropins, Pituitary, Serum Globulins, ALPHA GLOBULIN, BETA GLOBULIN, CHROMATOGRAPHY, GLYCOPROTEINS, GONADOTROPINS, PITUITARY, IMMUNOELECTROPHORESIS, MENOPAUSE, SERUM GLOBULINS, URINE