Fertility and Sterility, 33(3), 288-293
Twenty-five women scheduled for hysterectomy for nonmalignant disease participated in the study. Sperm storage in endocervical crypts was examined in three groups of patients: nine women pretreated with estrogen and inseminated with normal semen, nine women pretreated with gestagen and inseminated with normal semen, and seven women pretreated with estrogen and inseminated with abnormal semen. The number of crypts containing spermatozoa (colonized crypts) and the sperm density per crypt were examined in serially sectioned cervices. In estrogen-pretreated cervices both the percentage of colonized crypts and the sperm density were significantly higher than in gestagen-pretreated cervices. Large and giant crypts proved to be the main storage facility for spermatozoa. The localization of crypts along the endocervical canal did not influence sperm storage. The quality of semen appeared to be of critical importance to sperm storage. The percentage of colonized crypts and sperm density were severly reduced in patients inseminated with abnormal semen.
Insler, V., Glezerman, M., Zeidel, L., Bernstein, D., & Misgav, N. (1980). Sperm storage in the human cervix: a quantitative study. *Fertility and sterility*, *33*(3), 288-293. https://doi.org/10.1016/s0015-0282(16)44596-6
Insler V, Glezerman M, Zeidel L, Bernstein D, Misgav N. Sperm storage in the human cervix: a quantitative study. Fertil Steril. 1980;33(3):288-293. doi:10.1016/s0015-0282(16)44596-6
Insler, V., et al. "Sperm storage in the human cervix: a quantitative study." *Fertility and sterility*, vol. 33, no. 3, 1980, pp. 288-293.
Cervix Mucus/physiology, Cervix Uteri/drug Effects/physiology, Ethinyl Estradiol/pharmacology, Female, Humans, Insemination, Artificial, Male, Medroxyprogesterone/pharmacology, Sperm Count, Sperm Transport/drug Effects, Spermatozoa/physiology, Ethinyl Estradiol, Medroxyprogesterone, Artificial Insemination, Biology, Cervical Mucus, Cervix, Clinical Research, Comparative Studies, Contraception, Contraceptive Agents, Estrogen--administraction and Dosage, Contraceptive Agents, Female--administraction and Dosage, Contraceptive Agents, Progestin--administraction and Dosage, Contraceptive Agents--administraction and Dosage, Ethinyl Estradiol--administraction and Dosage, Evaluation, Family Planning, Genitalia, Genitalia, Female, Germ Cells, In Vitro, Medroxyprogesterone Acetate--administraction and Dosage, Mortality, Physiology, Reproduction, Reproductive Technologies, Research Methodology, Sperm Transport, Spermatozoa, Studies, Urogenital System, Uterus