A biorepository of maternal fluids from a preconceptional cohort for multiomics studies of ultra early human pregnancy

Understanding the embryo-mother interaction in humans from fertilization is a process that has not yet been elucidated. The use of animal models does not reflect the reality of ultra-early human pregnancy development. In vitro models, besides being incomplete due to the mother's absence, present ethical dilemmas in the use of human embryos for biomedical research. Currently, some studies analyze pregnancy from a multi-omics approach; However, these focus on the later stages of pregnancy. Therefore, the objective of this work is to generate a robust biorepository of female fluids at an ultra-early stage of pregnancy that allows for an in-depth study of the embryo-mother crosstalk. To our knowledge, the biorepository generated through this cohort is the first to integrally incorporate four maternal fluids for studying embryo-mother interaction in the ultra-early pregnancy period. The information obtained from multi-omics studies of the different fluids will allow the development of the human conception profile and its ultra-early development.