Male Fertility · Semen Analysis

Human cervical mucus can act in vitro as a selective barrier against spermatozoa carrying fragmented DNA and chromatin structural abnormalities

Bianchi PG, De Agostini A, Fournier J, Guidetti C, Tarozzi N, Bizzaro D, Manicardi GC

Published April 2004 Journal of assisted reproduction and genetics, 21(4), 97-102
DOI 10.1023/b:jarg.0000029492.54243.3c PMID 15270207 PMC PMC3455610

Abstract

Purpose

We have carried out experiments to determine if human cervical mucus can act as an in vitro selective barrier against spermatozoa morphologically normal that carry genetic structural abnormalities.

Methods

Sperm chromatin abnormalities have been evaluated by Chromomycin A3 and "endogenous" nick translation.

Results

The data obtained have shown that spermatozoa possessing higher levels of DNA protamination are more proficient in crossing the cervical mucus barrier. Moreover, the levels of positivity to endogenous nick translation treatment was practically zero in such spermatozoa.

Conclusions

We suggest that sperm penetration of cervical mucus could be used to select sperm preparations free of fragmented DNA or chromatin structural abnormalities for assisted reproduction.

Topics

Related research

Male Fertility › Semen Analysis › Sperm Function Testing
A De Agostini
PMID 15270207 15270207 DOI 10.1023/b:jarg.0000029492.54243.3c 10.1023/b:jarg.0000029492.54243.3c Bianchi et al. 2004, Bianchi 2004