Research Methods · Measurement and Statistics

Plasma progesterone levels in normal pregnancy, labor, and the puerperium. I. Method of assay

Yannone ME

Published August 15, 1968 American Journal of Obstetrics and Gynecology, 101(8), 1054-1057
DOI 10.1016/0002-9378(68)90347-5 PMID 5690916

Abstract

A method to measure plasma progesterone by gas-liquid chromatography is presented. The procedure allows for the preparation of a plasma progesterone extract clean enough for accurate submicrogram quantification by gas-liquid chromatography. In a gas chromatograph equipped with a hydrogen flame detector, the instrument modifications and operating conditions as outlined make possible the measurement of 0.001 μg (1 ng.) of progesterone standard on a stable recorder base line. In biologic samples the sensitivity of the assay is in the order of 0.01 μg (10 ng.) of progesterone.

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Research Methods › Measurement and Statistics › Instrument Development and Validation · Diagnostics › Hormone Testing › Serum Panels · Reproductive Endocrinology › Ovarian Hormones › Progesterone
Michael E. Yannone
Mike Yannone, M Yannone
PMID 5690916 5690916 DOI 10.1016/0002-9378(68)90347-5 10.1016/0002-9378(68)90347-5 Yannone et al. 1968, Yannone 1968