SUMMARY 1. The action of progesterone on the development of the mouse ovum from the eight-celled stage to the blastula has been studied in vitro . 2. Concentrations of progesterone below 2 μg/ml. produced no observable effect, whereas some toxicity was observed at 4 μg/ml. and few ova survived higher concentrations. 3. Herniation of the zona pellucida by the developing blastula is described; this process was inhibited by 4 μg/ml., but not by 2 μg/ml. of progesterone. 4. Blastulae appear to be more sensitive to the action of progesterone than are earlier stages. 5. Oestrogens afforded no demonstrable protection against the toxic action of progesterone when added to the culture medium. 6. The possible physiological significance of these findings is discussed.
PMID 13491736 13491736 DOI 10.1677/joe.0.0160080 10.1677/joe.0.0160080
Cite this article
Whitten, W. K. (1957). The effect of progesterone on the development of mouse eggs in vitro. The Journal of endocrinology, 16(1), 80-85. https://doi.org/10.1677/joe.0.0160080
Whitten WK. The effect of progesterone on the development of mouse eggs in vitro. J Endocrinol. 1957;16(1):80-85. doi:10.1677/joe.0.0160080
Whitten, W. K. "The effect of progesterone on the development of mouse eggs in vitro." The Journal of endocrinology, vol. 16, no. 1, 1957, pp. 80-85.
Keywords
Animals, In Vitro Techniques, Mice, Ovum/drug Effects, Progesterone/pharmacology, Progesterone, OVUM/effect of Drugs On, PROGESTERONE/effects
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