Abstract

Since the birth of Louise Brown in 1978, more than nine million children have been conceived using assisted reproductive technologies (ARTs). While the great majority of children are healthy, there are concerns about the potential epigenetic consequences of gametes and embryo manipulation. In fact, during the preimplantation period, major waves of epigenetic reprogramming occur. Epigenetic reprogramming is susceptible to environmental changes induced by ovarian stimulation, in-vitro fertilization, and embryo culture, as well as cryopreservation procedures. This review summarizes the evidence relating to oocytes and embryo cryopreservation and potential epigenetic regulation. Overall, it appears that the stress induced by vitrification, including osmotic shock, temperature and pH changes, and toxicity of cryoprotectants, might induce epigenetic and transcriptomic changes in oocytes and embryos. It is currently unclear if these changes will have potential consequences for the health of future offspring.

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Romualdo Sciorio, Patricia Fauque, Paolo Rinaudo
PMID 37445479 37445479 DOI 10.3390/jcm12134444 10.3390/jcm12134444 Sciorio et al. 2023, Sciorio 2023

Cite this article

Sciorio, R., Manna, C., Fauque, P., & Rinaudo, P. (2023). Can Cryopreservation in Assisted Reproductive Technology (ART) Induce Epigenetic Changes to Gametes and Embryos? Journal of clinical medicine. https://doi.org/10.3390/jcm12134444

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