Identification of two related pentapeptides from the brain with potent opiate agonist activity

J. Hughes; T. W. Smith; H. W. Kosterlitz; Linda A. Fothergill; B. A. Morgan; H. R. Morris

doi:10.1038/258577a0

Identification of two related pentapeptides from the brain with potent opiate agonist activity

Hughes J, Smith TW, Kosterlitz HW, Fothergill LA, Morgan BA, Morris HR

Published December 18, 1975 Nature, 258(5536), 577-580

DOI 10.1038/258577a0 PMID 1207728

Abstract

Enkephalin, a natural ligand for opiate receptors is composed of the pentapepides H-Tyr-Gly-Gly-Phe-Met-OH and H-Tyr-Gly-Gly-Phe-Leu-OH. The evidence is based on the determination of the amino acid sequence of natural enkephalin by the dansyl-Edman procedure and by mass spectrometry followed by synthesis and comparison of the natural and synthetic peptides.

Linda A. Fothergill

L Fothergill

PMID 1207728 1207728 DOI 10.1038/258577a0 10.1038/258577a0 Hughes et al. 1975, Hughes 1975

Keywords

Amino Acid Sequence, Animals, Brain/metabolism, Brain Chemistry, Ligands/analysis, Muscle Contraction/drug Effects, Naloxone/pharmacology, Nerve Tissue Proteins/analysis/pharmacology, Oligopeptides/analysis/pharmacology, Opium/metabolism, Receptors, Drug, Swine, Ligands, Nerve Tissue Proteins, Oligopeptides, Receptors, Drug, Naloxone, Opium