PURIFICATION OF GONADOTROPHIN FROM HUMAN MENOPAUSAL URINE

Donini P; Puzzuoli D; Montezemolo R

doi:10.1530/acta.0.0450321

Reproductive Endocrinology · Ovulation Physiology

PURIFICATION OF GONADOTROPHIN FROM HUMAN MENOPAUSAL URINE

Donini P, Puzzuoli D, Montezemolo R

Published March 1964 Acta Endocrinologica, 45(3), 321-328

DOI 10.1530/acta.0.0450321 PMID 14131763

Abstract

Gonadotrophins were prepared from human postmenopausal urine with the kaolin-acetone method and purified by adsorption of impurities on diethylamine ethyl cellulose (DEAE-C). The last step, namely chromatography on the column of permutit, resulted in a significantly improved specific activity. The immunoelectrophoresis showed that the purified HMG is not homogeneous and that the constituents are glycoproteins which migrate into the region α1, α2 and β globulins.

Topics

Reproductive Endocrinology Ovulation Physiology Follicular Development

Reproductive Endocrinology › Ovulation Physiology › Follicular Development

PMID 14131763 14131763 DOI 10.1530/acta.0.0450321 10.1530/acta.0.0450321 Donini et al. 1964, Donini 1964

Keywords

Alpha-Globulins, Beta-Globulins, Chromatography, Glycoproteins, Gonadotropins, Gonadotropins, Pituitary, Immunoelectrophoresis, Menopause, Serum Globulins, Urine, Alpha-Globulins, Beta-Globulins, Glycoproteins, Gonadotropins, Gonadotropins, Pituitary, Serum Globulins, ALPHA GLOBULIN, BETA GLOBULIN, CHROMATOGRAPHY, GLYCOPROTEINS, GONADOTROPINS, PITUITARY, IMMUNOELECTROPHORESIS, MENOPAUSE, SERUM GLOBULINS, URINE

PURIFICATION OF GONADOTROPHIN FROM HUMAN MENOPAUSAL URINE

Abstract

Topics

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