Stereospecific and nonspecific interactions of the morphine congener levorphanol in subcellular fractions of mouse brain

Avram Goldstein; Louise I. Lowney; B. K. Pal

doi:10.1073/pnas.68.8.1742

Research Methods · Preclinical Research

Stereospecific and nonspecific interactions of the morphine congener levorphanol in subcellular fractions of mouse brain

Goldstein A, Lowney LI, Pal BK

Published August 1971 Proceedings of the National Academy of Sciences of the United States of America, 68(8), 1742-1747

DOI 10.1073/pnas.68.8.1742 PMID 5288759 PMC PMC389284

Abstract

A METHOD IS DESCRIBED FOR ANALYZING THE ASSOCIATION OF THE OPIATE NARCOTIC LEVORPHANOL WITH BRAIN TISSUE INTO THREE COMPONENTS: nonsaturable, saturable nonspecific, and saturable stereospecific. The method may be of general applicability for the study of the interaction of drugs with body tissues. In mouse brain the stereospecific binding of levorphanol represents only 2% of the total association of drug with tissue, and it was found only in certain membrane fractions. The material responsible for the stereospecific binding might be the opiate receptor.

Topics

Research Methods Preclinical Research Laboratory Methods

Research Methods › Preclinical Research › Laboratory Methods

Avram Goldstein, Louise I. Lowney

A Goldstein, L Lowney

PMID 5288759 5288759 DOI 10.1073/pnas.68.8.1742 10.1073/pnas.68.8.1742 Goldstein et al. 1971, Goldstein 1971

Keywords

Animals, Autoradiography, Binding Sites, Brain/cytology/metabolism, Brain Chemistry, Carbon Isotopes, Cell Fractionation, Cell Membrane/analysis, Cell Nucleus/analysis, Centrifugation, Density Gradient, Chromatography, DEAE-Cellulose, Chromatography, Gel, Dialysis, Levorphanol/analysis/metabolism, Male, Mice, Mitochondria/analysis, Receptors, Drug, Stereoisomerism, Tritium, Carbon Isotopes, Receptors, Drug, Tritium, Levorphanol

Stereospecific and nonspecific interactions of the morphine congener levorphanol in subcellular fractions of mouse brain

Abstract

Topics

By this author

Dynorphin-(1-13), an extraordinarily potent opioid peptide

Keywords