The Structure of Water in the Myometrium

STUDIES ON THE STORAGE OF THE OESTROGEN-PRECURSOR IN THE INTERSTITIAL GLAND OF THE OVARY OF RATS TREATED WITH OESTRADIOL BENZOATE AND PROGESTERONE

Aldman B et al., 2009 · Acta Anatomica

Some preliminary observations on the proton magnetic resonance in biologic samples

Odeblad E et al., 2008 · Acta radiologica. Supplement

Morphological characterization of different human cervical mucus types using light and scanning electron microscopy

Menárguez M et al., 2003 · Human reproduction (Oxford, England)

This study was conducted on human cervical mucus using light microscopy (LM) and scanning electron microscopy (SEM). The objective was the morphological characterization of the different mucus types, with samples taken from the lumen of the cervix and from the different secretory zones of the cervical mucosa. A total of 230 samples from 195 women were spread out on slides and air dried. The phenomenon of 'ferning' was observed and assessed in these samples using both LM and SEM. Further samples from the lumen of the cervix and the different secretory crypts were spread out on cover slips and fixed with glutaraldehyde (2.5%) to be studied by SEM. The results show the presence of four different morphological mucus types, namely L, S, P and G, in both types of sample using dried and fixed techniques. Mucus from the lumen of the cervix appears to be a morphologically heterogeneous entity. It contains different types of secretions, the proportions of which vary throughout the menstrual cycle. The different mucosal types show different types of crystallization, different patterns of ultrastructure (probably related to the arrangement of the glycoprotein network) and are produced in different secretory zones of the crypts in the cervix.

Cervical mucus and their functions

Odeblad E, 1997 · Journal of the Irish Colleges of Physicians and Surgeons

PROTON MAGNETIC RESONANCE STUDIES ON THE STRUCTURE OF WATER IN THE MYOMETRIUM

ODEBLAD E et al., 1965 · Acta obstetricia et gynecologica Scandinavica

High affinity progesterone binding sites of human uterine microsomal membranes

Haukkamaa M, 1984 · J Steroid Biochem

Microsomal membranes sedimented at 40 000 g were prepared from human myometrium samples. The progesterone binding properties of microsomal suspensions were determined by incubating microsomes and [3H]progesterone at 4 degrees C. Dextran-coated charcoal was used for the separation of bound and free steroids. Membrane-associated progesterone binding sites of high affinity were identified in microsomes prepared from pregnant and nonpregnant uteri. The binding was saturable (Kd approximately 4 X 10(-9) M, concentration of binding sites 400-900 fmol/mg microsomal protein) and specific for natural progesterone. Of 21 steroids tested only 21-hydroxy-4-pregnene-3,20-dione, 17 alpha-hydroxyprogesterone and testosterone showed moderate competition against progesterone with relative affinities between 7.0-20.0% (R.A. of progesterone 100%). 5 alpha-Dihydroprogesterone and 5 alpha-dihydrotestosterone showed weak cross reaction (relative affinities 2.5 and 2.0%, respectively). Corticosteroids, estrogens and the 5 synthetic progestins tested showed only weak competition with relative affinities lower than 1.0%. These microsomal progesterone binding sites of high affinity and limited capacity resemble steroid hormone receptors but they are different from the soluble cytosolic progesterone receptor of human uterus in terms of steroid specificity. The physiological function of this microsomal progesterone receptor is unknown.

Some Investigations with Nuclear Magnetic Resonance on Water Associated with the Vaginal Cells*

Odeblad E, 1960 · Acta Obstetricia et Gynecologica Scandinavica

The pathophysiology of uterine adenomyosis: an update

Benagiano G et al., 2012 · Fertility and Sterility